In this process, under specific buffer conditions, nucleic acids (DNA/RNA) specifically bind to silica or glass fiber membranes, while impurities such as proteins and polysaccharides are washed away. The purified nucleic acids are then eluted from the membrane, allowing for the extraction of high-purity DNA/RNA from samples. The extracted purified nucleic acids can be used in various common downstream experiments, such as enzyme digestion, reverse transcription, PCR, ligation, transformation, RT-PCR, library construction, sequencing, in vitro translation, and Southern blotting.
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Enquiry only. No payment is taken.Application:
It is widely used in scientific research, medical diagnosis, animal husbandry, agriculture, bio-industry and public health.
Features:
1. High nucleic acid purity: The silica membrane specifically binds to nucleic acids under high salt conditions, efficiently removing proteins, polysaccharides, lipids, and PCR inhibitors.
2. No risk of cross-contamination: All steps are completed within the centrifuge column-collection tube system; waste liquid is passed through the membrane and discarded after each step via centrifugation.
3. No special equipment required: Only a standard benchtop centrifuge is needed.
Extraction Process:
Sample treatment with lysis buffer—nucleic acid binding to membrane—washing—elution of nucleic acid from membrane.
Advantages:
1. High concentration, high purity, and strong stability of extracted nucleic acids;
2. Convenient operation, no need for phenol-chloroform extraction, safe and environmentally friendly.
Parameters:
| Item No. | Model | Specification | Applicable Sample Types | Nucleic Acid Types Extracted |
|
DR1161 |
Z1 |
50T/Kit; 200T/Kit |
Blood/cell/tissue samples |
Genomic DNA |
| Z2 | Fresh or frozen plant tissues and plant cells | |||
| Z3 | Various bacterial solutions | |||
| Z4 | Animal tissues preserved at -80℃ or fresh, cultured cells, suspension cells |
Total RNA |
||
| Z5 | Plants stored at -80℃ or fresh | |||
| Z6 | Culture medium containing the target plasmid | Plasmid DNA | ||
| Z7 | Agarose gel containing the target nucleic acid fragment | Single-stranded, double-stranded, or circular DNA | ||
| Package Information |
20 Kits/Carton (or 40 Kits/Carton) |
|||
| Package Size（W*D*H) |
740*420*300mm (or 580*550*580mm) |
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| Gross Weight |
10kg (or 18kg) |
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