High Performance Liquid Chromatography

High Performance Liquid Chromatography System BK-100HPLC

Model BK-100HPLC

Liquid chromatography is a technique that uses a liquid as the mobile phase to separate mixtures through a stationary phase. It utilizes the differences in partition coefficients and adsorption capacities of various components between the two phases, achieves separation through repeated distribution equilibrium, and combines with detectors for qualitative and quantitative analysis.

Max. load
Standard tray 2ml*54…
Noise
≤0.25x10⁻⁵AU /±1x10⁻⁵…
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A closer look

About this equipment

Features:

1. The system offers a comprehensive and flexible isocratic mode, and a binary gradient system is also available. Detection capabilities include DAD, UV/Vis, FL, RI and ELSD.

2. The new optional, solvent manager units can easily switch 4 to 8 kinds of solvent base on binary gradient system. There are several configurations available for user selection.

3. The real-time solvent pressure compensation control enhances detection precision. The integrated check valve ensures stable fluid delivery and simplifies maintenance.

LC-100 HP High-Pressure Constant Flow Pump:

Liquid Infusion System Dual-piston reciprocating parallel pump, equipped with a binary high-pressure mixer
Flow Rate Range 0.001~10ml/min
Max Output Pressure 42MPa
Flow Accuracy ≤±1%
Flow Precision RSD<0.07%
Qualitative Repeatability RSD6≤0.1%(naphthalene/methanol standards)
Quantitative Repeatability RSD6≤0.3%(naphthalene/methanol standards)
Pressure Fluctuation 0.1MPa
Power Supply AC220/110V, 50/60Hz

LC-100 UV Detector:

Flow Cell Volume 8.0μl
Light Source D2 lamp
Wavelength Range 190~680nm
Spectrum Bandwidth 8nm
Wavelength Accuracy ±1nm
Wavelength Precision Below 0.1nm
Noise ≤0.25×10⁻⁵AU(static)/±1×10⁻⁵AU(dynamic, under given conditions)
Drift ≤0.4×10⁻³AU/h(static)/≤2×10⁻³AU/h(dynamic, under given conditions)
Minimum Detectable Concentration 3×10⁻⁹g/ml(naphthalene/methanol solution)

LC-100 PDA Detector:

Wavelength Range 200~900nm(200~600*), Above 600nm just for W lamp
Arrays 512
Spectrum Bandwidth 10nm
Light Source D2 lamp+W lamp
Noise ±5*10⁻⁵AU
Sample Rate Max100Hz
Interface LAN, USB, R232

ARCUS 5 Auto-sampler:

Sample Loading Standard tray (1) 2ml*54 vials(default standard configuration)
Expanded tray (2) 10ml*15 vials (3) 4ml*35 vials
Each side could be set with one of the tray types and the tray in one side could be different from the other side; the system could automatically identify the tray type; maximum 108 vials are available(2ml vial)
Vial Height H≤52mm(including septa and cap-mat)
Metering Pump Volume 200μl(precision: 0.1μl)
Injection Mode Full loop injection

Partial loop injection

No waste injection
Automatic Protection Vial-missing alarm, sample needle fault alarm, pipeline jammed alarm, and leakage alarm
Power Supply AC115~230V, 50/60Hz
System Test Quantitative Repeatability Full loop injection: RSD6≤0.3%
Partial loop injection: RSD6≤0.5%(injection volume ≥ 10μl)

No waste injection: RSD6≤1.0%(injection volume ≥ 10μl)
Memory Effect ≤0.005%(designated washing procedure)
Maximum Pressure 40Mpa(conventional) 69Mpa(ultra-high performance system)

Technical Parameters:

Model BK-100HPLC
(Type I)
Isocratic manual system		 BK-100HPLC
(Type II)
Gradient manual system		 BK-100HPLC
(Type III)
Isocratic manual system		 BK-100HPLC
(Type IV)
Gradient manual system		 BK-100HPLC
(Type V)

Binary gradient manual system with PDA
Software With Audit Trail / / Yes Yes Yes
Diode Array Detector / / / / Yes
High-pressure Constant Flow Pump LC-100 HP*1 LC-100 HP*2 LC-100 HP*1 LC-100 HP*2 LC-100 HP*2
UV Detector
LC-100 UV		 Yes Yes Yes Yes /
Diode Array Detector (With English Workstation) / / / / LC-100 PDA*1
Workstation LC-100 WS Yes Yes / / /
Workstation EX-Clarity C50 / / Yes Yes /
Manual Sampler 7725i Yes Yes Yes Yes Yes
C18 5μm ID4.6*250mm Yes Yes Yes Yes Yes
High-precision Mixer EX1600 MX3 / Yes / Yes Yes
Power Supply

AC220/110V, 50/60Hz
Optional Accessory

UV detector, RI detector, PDA detector, ELSD detector, FLD100 detector, column, LC-CO 100 standard column oven, ARCUS 5 auto-sampler
Package Size (W*D*H) 450*620*350mm*2 450*620*350mm*3 450*620*350mm*2 450*620*350mm*3 450*620*350mm*2

660*560*500mm*1
Gross Weight 19kg;

15kg

 19kg;

19kg;

15kg

 19kg;

15kg

 19kg;

19kg;

15kg

 19kg;

19kg;

18kg
The details

Specifications

Liquid Infusion System
Dual-piston reciprocating parallel pump, equipped with a binary high-pressure mixer
Flow Rate Range
0.001~10ml/min
Max Output Pressure
42MPa
Flow Accuracy
≤±1%
Flow Precision
RSD<0.07%
Qualitative Repeatability
RSD6≤0.1%(naphthalene/methanol standards)
Quantitative Repeatability
RSD6≤0.3%(naphthalene/methanol standards)
Pressure Fluctuation
0.1MPa
Power Supply
AC220/110V, 50/60Hz
Flow Cell Volume
8.0μl
Light Source
D2 lamp
Wavelength Range
190~680nm
Spectrum Bandwidth
8nm
Wavelength Accuracy
±1nm
Wavelength Precision
Below 0.1nm
Noise
≤0.25x10⁻⁵AU(static)/±1x10⁻⁵AU(dynamic, under given conditions)
Drift
≤0.4x10⁻³AU/h(static)/≤2x10⁻³AU/h(dynamic, under given conditions)
Minimum Detectable Concentration
3x10⁻⁹g/ml(naphthalene/methanol solution)
Wavelength Range
200~900nm(200~600*), Above 600nm just for W lamp
Arrays
512
Spectrum Bandwidth
10nm
Light Source
D2 lamp+W lamp
Noise
±5*10⁻⁵AU
Sample Rate
Max100Hz
Interface
LAN, USB, R232
Sample Loading
Standard tray (1) 2ml*54 vials(default standard configuration)Expanded tray (2) 10ml*15 vials (3) 4ml*35 vialsEach side could be set with one of the tray types and the tray in one side could be different from the other side; the system could automatically identify the tray type; maximum 108 vials are available(2ml vial)
Vial Height
H≤52mm(including septa and cap-mat)
Metering Pump Volume
200μl(precision: 0.1μl)
Injection Mode
Full loop injectionPartial loop injectionNo waste injection
Automatic Protection
Vial-missing alarm, sample needle fault alarm, pipeline jammed alarm, and leakage alarm
Power Supply
AC115~230V, 50/60Hz
System Test Quantitative Repeatability
Full loop injection: RSD6≤0.3%Partial loop injection: RSD6≤0.5%(injection volume ≥ 10μl)No waste injection: RSD6≤1.0%(injection volume ≥ 10μl)
Memory Effect
≤0.005%(designated washing procedure)
Maximum Pressure
40Mpa(conventional) 69Mpa(ultra-high performance system)
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