Nucleic Acid Extractor

Real-time PCR System

Model LEIA-X4 LEIA-X6 Microplate & PCR

Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Net weight
28kg
Dimensions
375*505*375mm
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A closer look

About this equipment

Application:

It can be widely used in infectious disease research, food pathogen detection, waterborne pathogen detection, drug analysis, stem cell research, pharmacogenomics research, tumor and genetic disease research, plant science and agricultural biotechnology.

Working Principle:

The temperature step change is controlled by the semiconductor Peltier to realize PCR amplification. Use high-sensitivity PD unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control X and Y axis movement to achieve 96-hole scanning. Finally, accurate analysis is carried out through powerful software.

Features:

1. Excellent temperature control performance of the instrument, Module Max heating rate heating rate 7.0℃/s.

2. No edge effect, no optical path correction, top excitation/detection, non-contact measurement.

3. Fluorescence detection adopts PD sensor with high sensitivity.

4. Long-life LED light source, stable emission wavelength, maintenance-free.

5. 4/6-channel fluorescence detection, no cross interference between channels.

6. User-friendly and fully functional software, flexible program setting, comprehensive analysis and reporting functions, all the parameters can be stored.

Real-time PCR Hardware:

Thermoelectric cooling module (TEM) is a semiconductor device composed of many tiny and effective heat pumps. By applying a low-voltage DC power supply, heat will be transferred from one side of the TEM to the other side, resulting in a phenomenon that one side of the TEM becomes hot and the other side becomes cold. Since this phenomenon is completely reversible, when the polarity of the DC power supply is changed, it will be the opposite. This product adopts a long-life series TEM, which provides longer life and more efficiency during thermal cycling.

The integrated design of the scanning module and the heating cover module, relying on its own gravity to compress the heating plate and the reagent cover, and is supported by four compression springs to prevent the sample tube from being crushed by four; at the same time, the rubber pad around the heating cover is pressed to ensure that there is no external light source interference in the detection ; The bottom of the cam mechanism is used to support the space to ensure the smooth sliding of the heating module; the guide rail mechanism on both sides of the heating module prevents the module from shifting and ensures the accuracy of the mechanical scanning structure.

Real-time PCR Software:

The software includes functions such as absolute quantification experiment, melting curve experiment, relative quantification(AACT) experiment, and genotyping experiment. Enter the attribute setting interface and select different function modules. Guided flow operation, convenient for users to quickly complete experimental settings. The software can open the recent experiment record template for easy viewing of recent experiments and the creation of new experiments.

Technical Parameters:

Model LEIA-X4

LEIA-X6

Sample Capacity

96*0.1ml PCR plate, 12*8-strip tubes, 96*0.1ml single tube (Transparent Cover)

Reaction System

5~50μl

Dynamics Range

1~10¹⁰ copies

Channel

4

6
Emission Light

LED

Detector

PD

Detection Path F1: FAM/SYBR GREEN;

F2: VIC/JOE/HEX/TET;

F3: ROX/TEXAS-RED;

F4: Cy5

F1: FAM/SYBR GREEN;

F2: VIC/JOE/HEX/TET;

F3:CY3;

F4: ROX/TEXAS RED;

F5: CY5;

F6: CY5.5

Excitation Wavelength

455~650nm

455~685nm

Detection Wavelength

510~710nm

510~740nm

Fluorescence Detection Repeatability

CV≤3%

Fluorescence Detection Accuracy

CV≤5%

Fluorescence Detection Linearity

r≥0.995

Module Temp. Range

4~105℃

Ramp Rate

7.0℃/s(max)

Temp. Accuracy

±0.5℃

Temp. Uniformity

≤±1℃

Temp. Control Mode

Block and tube

Gradient Temp. Range

It can be set within the temperature range of 30°C to 105°C, with a temperature gradient span of 1°C to 36°C

Hot-Lid Temp. Range

100℃, automatic hot-lid

Scanning Mode

Top scanning

Operation Mode

Continuous

Scanning Time

8.5s

Special Function

Absolute quantitative automatic analysis, relative quantification, SNP Analysis, melting curve analysis, etc.

Operation System

Microsoft: Windows 10

Port Method

USB Port

Power Supply

AC100~240V, 50/60Hz

Dimension(W*D*H) 375*505*375mm
Net Weight

28kg

Packing Size(W*D*H)

645*565*605mm

Gross Weight

49kg

The details

Specifications

Model
LEIA-X4 / LEIA-X6
Sample Capacity
96*0.1ml PCR plate, 12*8-strip tubes, 96*0.1ml single tube (Transparent Cover)
Reaction System
5~50μl
Dynamics Range
1~10¹⁰ copies
Channel
4 / 6
Emission Light
LED
Detector
PD
Detection Path
F1: FAM/SYBR GREEN;F2: VIC/JOE/HEX/TET;F3: ROX/TEXAS-RED;F4: Cy5 / F1: FAM/SYBR GREEN;F2: VIC/JOE/HEX/TET;F3:CY3;F4: ROX/TEXAS RED;F5: CY5;F6: CY5.5
Excitation Wavelength
455~650nm / 455~685nm
Detection Wavelength
510~710nm / 510~740nm
Fluorescence Detection Repeatability
CV≤3%
Fluorescence Detection Accuracy
CV≤5%
Fluorescence Detection Linearity
r≥0.995
Module Temp. Range
4~105℃
Ramp Rate
7.0℃/s(max)
Temp. Accuracy
±0.5℃
Temp. Uniformity
≤±1℃
Temp. Control Mode
Block and tube
Gradient Temp. Range
It can be set within the temperature range of 30°C to 105°C, with a temperature gradient span of 1°C to 36°C
Hot-Lid Temp. Range
100℃, automatic hot-lid
Scanning Mode
Top scanning
Operation Mode
Continuous
Scanning Time
8.5s
Special Function
Absolute quantitative automatic analysis, relative quantification, SNP Analysis, melting curve analysis, etc.
Operation System
Microsoft: Windows 10
Port Method
USB Port
Power Supply
AC100~240V, 50/60Hz
Dimension(W*D*H)
375*505*375mm
Net Weight
28kg
Packing Size(W*D*H)
645*565*605mm
Gross Weight
49kg
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  2. 02Confirm the details

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  3. 03We source and ship

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